Archive




Volume 3, Issue 6, December 2014, Page: 464-468
Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)
Yohannes Seyoum, Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia
Firew Mekbib, School of Plant sciences, Haramaya University (HU), Dire-Dewa, Ethiopia
Adefris Teklewold, Crop Research Directorate, Ethiopian Institute of Agriculture Research (EIAR), Addis Ababa, Ethioipia
Belayneh Admassu, Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia
Dawit Beyene, Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia
Zelalem Fisseha, Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia
Received: Nov. 23, 2014;       Accepted: Dec. 11, 2014;       Published: Dec. 22, 2014
DOI: 10.11648/j.aff.20140306.15      View  3832      Downloads  246
Abstract
‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security roles to the pastoralist of Somali Region State in Ethiopia. However, the plant is threatened with extinction due to over exploitation and its’ poor natural regeneration capacity. The aim of this was to explore the potential for in vitro rapid regeneration of ‘yeheb’ from cotyledon and leaf explants on Murashige and Skoog (MS) media supplemented with 1.0 – 8.0 mg l-1concentrations of 2, 4-D for callus induction and 2.0 and 3.0 mg l-1concentration of N6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (Kin) with combination of 4.0 mg l-1 of 2, 4-Dichlorophenyl acetic acid (2, 4-D) for embryo induction. The result of these studies revealed that the highest percentage of callus induction (89%) were obtained from both leaf and cotyledon explants on MS media supplemented with 4.00 and 8.00 mg l-1 2, 4-D, respectively. The highest percentage of embryo regeneration responses (88.89 and 77.78%) were obtained from leaf and cotyledon explants on same media: MS media supplemented by 3.00 mg l-1 TDZ +4.00 mg l-1 2, 4-D. As a conclusion; this is the first attempt for callus and embryo in vitro regeneration of C. edulis and permissible result for mass propagation and cryopreservation.
Keywords
Callus, Cotyledon, Embryo, Explants, In Vitro
To cite this article
Yohannes Seyoum, Firew Mekbib, Adefris Teklewold, Belayneh Admassu, Dawit Beyene, Zelalem Fisseha, Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl), Agriculture, Forestry and Fisheries. Vol. 3, No. 6, 2014, pp. 464-468. doi: 10.11648/j.aff.20140306.15
Reference
[1]
Ali, H.M., 1988. Cordeauxia edulis: Production and Forage Quality in Central Somalia. Thesis for the degree of Master of Science in Rangeland Resources, National University of Somalia, Somalia.
[2]
Teketay, D. and Eshete, A., 2004. Status of indigenous fruits in Ethiopia. In: Chikamai B, Eyog-Matig O, Mbogga M (eds.) Review and Appraisal on the Status of Indigenous Fruits in Eastern Africa: A Report Prepared for IPGRI-SAFORGEN in the Framework of AFRENA/FORENESSA, Kenya Forestry Research Institute, Nairobi, Kenya, pp 3-35.
[3]
Miège, J. and Miège, M.N., 1978 Cordeauxia edulis a Caesalpinaceae of Arid Zones of East Africa, Caryologic, blastogenic and biochemical features. Potential aspects for nutrition. Economic Botany, 32: 336-345.
[4]
Booth, F.E.M. and Wickens, G.E., 1988. Non-timber uses of selected arid zone trees and shrubs in Africa. FAO Conservation Guide 19, 52-58.
[5]
Brink, M., 2006. Cordeauxia edulis Hemsl Record from Protobase. PROTA (Plant resources of tropical Africa / Ressourcesvégétales de l’Afriquetropicale), Wageningen, Netherlands http://database.prota.org/search.htm (Accessed on September 14, 2011)
[6]
N.A.S (National Academy of Science), 1979. Tropical legumes: Resource for the Future, Nat Acad. Sci. Washington DC, 261 pp.
[7]
FAO, 1988. Traditional food plants, Food and nutrition paper 42:224-27.
[8]
Assefa, F., Bollini, R. and Kleiner, D., 1997. Agricultural potential of little used tropical legumes with special emphasis on Cordeauxia edulis (Ye-eb nut) and Sphenostylis stenocarpa (African yam bean). Giessener Beiträgezur Entwicklungsforschung, 24:237–242.
[9]
Bally, P.R.O., 1966. Miscellaneous notes on the flora of Tropical East Africa, 29. Enquiry into the occurrence of the Yeheb nut (Cordeauxia edulis Hemsl.) in the Horn of Africa. Candollea 21 (1), 3-11.
[10]
Hemming, C.F., 1972. The vegetation of the northern region of Somalia Republic. Proceeding of Linnaeus Social London, 177:173-250.
[11]
Drechsel, P. and Zech, W., 1988. Site conditions and nutrient status of Cordeauxia edulis (Caesalpiniaceae) in its natural habitat in central Somalia.Economic Botany, 42: 242–249.
[12]
Mussa, M., 2010.Cordeauxia edulis (Yeheb): resource status, utilization and management in Ethiopia. Thesis for the degree of Philosophiae Doctor, University of Wales.
[13]
Fatima, B.,Usman M., Ashraf, T., Waseem, R. and Ali, M.A., 2007. In vitro shoot regeneration from cotyledon and hypocotyl explants of dahlia cultivars. Pak. J. Agri. Sci.,44(2):312-316
[14]
Al-Naggar, A.M.M., Shabana, R., Rady,M.R.,Ghanem, S.A., Saker, M.M., Reda, A.A., MatterM.A., and Eid, S.A.M., 2010. In vitro callus initiation and regeneration in some canola varieties. International Journal of Academic Research 2(6): 357-362
[15]
Sié, R.S., Charles, G.,,Sakhanokho, H.F., Toueix, Y., Djè ,Y., Sangaré, A. and Branchard, M., 2010. Protocols for callus and somatic embryo initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of explant type, sugar, and plant growth regulators. Australian Journal of Crop Science 4(2):98-106.
[16]
SAS Institute Inc., 2002. Statistical Analysis Software, Version 9.0. Cary, North Carolina, USA.
[17]
Rao, S., Patil, P. and Kaviraj, C.P., 2005. Callus induction and organogenesis from various explants in Vigna radiata (L.) Wilczek. Indian Journal of Biotechnology, 4:556-560.
[18]
Amoo, S.O. and Ayisire, B.E., 2005. Induction of callus and somatic embryogenesis from cotyledon explants of Parkiabiglobosa (Jacq.) Benth.African Journal of Biotechnology, 4(1):68-71.
[19]
Du, N., and Pijut, P.M., 2008. Regeneration of plants from Fraxinuspennsylvanica hypocotyls and cotyledons. Scientia Horticulturae, 118:74–79.
[20]
Warren, G., 1991. The regeneration of plants from cultured cells and tissues. In: Plant Cell and Tissue Culture. A. Stafford and G. Warren (Eds.) p. 85.
[21]
Rita, I. and Floh, E.I.S., 1995. Tissue culture and micro propagation of Cupheaericoides, a potential source of medium- chain fatty acids. Plant Cell, Tissue and Organ Culture, 40:187-189.
Browse journals by subject