Volume 3, Issue 6, December 2014, Page: 464-468
Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl)
Yohannes Seyoum, Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia
Firew Mekbib, School of Plant sciences, Haramaya University (HU), Dire-Dewa, Ethiopia
Adefris Teklewold, Crop Research Directorate, Ethiopian Institute of Agriculture Research (EIAR), Addis Ababa, Ethioipia
Belayneh Admassu, Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia
Dawit Beyene, Holetta biotech Laboratory, Ethiopian Institute of Agriculture Research (EIAR), Holetta, Ethiopia
Zelalem Fisseha, Dryland Crop Research Department, Somali Region Pastoral and Agro-pastoral Research Institute (SoRPARI), Jijiga, Ethiopia
Received: Nov. 23, 2014;       Accepted: Dec. 11, 2014;       Published: Dec. 22, 2014
DOI: 10.11648/j.aff.20140306.15      View  3832      Downloads  246
‘Yeheb’ (Cordeauxia edulis Hemsl) is a multipurpose and evergreen shrub and endemic to southeastern corner of Ethiopia and Somalia. It is adapted to low and irregular rainfall and survives a very long dry season. It has enormous economic and food security roles to the pastoralist of Somali Region State in Ethiopia. However, the plant is threatened with extinction due to over exploitation and its’ poor natural regeneration capacity. The aim of this was to explore the potential for in vitro rapid regeneration of ‘yeheb’ from cotyledon and leaf explants on Murashige and Skoog (MS) media supplemented with 1.0 – 8.0 mg l-1concentrations of 2, 4-D for callus induction and 2.0 and 3.0 mg l-1concentration of N6-benzylaminopurine (BAP), thidiazuron (TDZ) and kinetin (Kin) with combination of 4.0 mg l-1 of 2, 4-Dichlorophenyl acetic acid (2, 4-D) for embryo induction. The result of these studies revealed that the highest percentage of callus induction (89%) were obtained from both leaf and cotyledon explants on MS media supplemented with 4.00 and 8.00 mg l-1 2, 4-D, respectively. The highest percentage of embryo regeneration responses (88.89 and 77.78%) were obtained from leaf and cotyledon explants on same media: MS media supplemented by 3.00 mg l-1 TDZ +4.00 mg l-1 2, 4-D. As a conclusion; this is the first attempt for callus and embryo in vitro regeneration of C. edulis and permissible result for mass propagation and cryopreservation.
Callus, Cotyledon, Embryo, Explants, In Vitro
To cite this article
Yohannes Seyoum, Firew Mekbib, Adefris Teklewold, Belayneh Admassu, Dawit Beyene, Zelalem Fisseha, Induction of Callus and Somatic Embryogenesis from Cotyledon and Leaf Explants of Yeheb (Cordeauxia edulis Hemsl), Agriculture, Forestry and Fisheries. Vol. 3, No. 6, 2014, pp. 464-468. doi: 10.11648/j.aff.20140306.15
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