This study evaluated the effect of lactic acid bacteria (LAB) on fermentation and aflatoxin concentrations of napier grass, whole crop green maize and whole crop dry maize plant silages, infested with aflatoxigenic mold Aspergillus flavus. The forage was chopped into 10kg portions in duplicates and inoculated with 100 ml of 106-7 CFU/ml, B2 27 Lb. plantarum/pentosus/paraplantarum (LAB1); B410Lb. plantarum/pentosus/paraplantarum (LAB 2); LAB 1 + 100 ml of cultured spores (100 cfu/ml) of mold; LAB 2 + 100 ml of cultured spores (100 cfu/ml) of mold; control 1- no addition; control 2 – mold only; control 3 – LAB 1 only; control 4 – LAB 2 only. The inoculated silage bags were sealed for 90 days and sampling done monthly to evaluate LAB, fungal counts and aflatoxin B1. Dry matter (DM), pH, crude protein (CP), neutral detergent fiber (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) were determined using AOAC methods. Mold population decreased in month one but increased thereafter. Silage type determined LAB (p = 6.2e-08) and mold (p= 3.9e-08) proliferation. Dry maize and napier grass silages favoured LAB and mold growth, respectively. LAB inhibited mold growth and aflatoxin production (p= 2.2e-04). Silage pH in the third month ranged between 4.55 – 5.67 with no smell of butyric acid. Though napier and dry maize plants showed higher nutritional qualities, they favoured mold growth and aflatoxin proliferation. Aflatoxin-inhibiting LAB starters are useful in controlling aflatoxin risk in silage.
| Published in | Agriculture, Forestry and Fisheries (Volume 12, Issue 1) |
| DOI | 10.11648/j.aff.20231201.14 |
| Page(s) | 23-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Aflatoxin, Aspergillus Flavus, Lactic Acid Bacteria
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APA Style
Sheila Okoth, Leonard Wamae, Innocent Kariuki, Limbikani Matumba, Vesa Joutsjoki, et al. (2023). Evaluation of Lactic Acid Bacteria for the Control of Aflatoxin Contamination in Silage. Agriculture, Forestry and Fisheries, 12(1), 23-31. https://doi.org/10.11648/j.aff.20231201.14
ACS Style
Sheila Okoth; Leonard Wamae; Innocent Kariuki; Limbikani Matumba; Vesa Joutsjoki, et al. Evaluation of Lactic Acid Bacteria for the Control of Aflatoxin Contamination in Silage. Agric. For. Fish. 2023, 12(1), 23-31. doi: 10.11648/j.aff.20231201.14
AMA Style
Sheila Okoth, Leonard Wamae, Innocent Kariuki, Limbikani Matumba, Vesa Joutsjoki, et al. Evaluation of Lactic Acid Bacteria for the Control of Aflatoxin Contamination in Silage. Agric For Fish. 2023;12(1):23-31. doi: 10.11648/j.aff.20231201.14
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Download@article{10.11648/j.aff.20231201.14,
author = {Sheila Okoth and Leonard Wamae and Innocent Kariuki and Limbikani Matumba and Vesa Joutsjoki and Hannu Korhonen},
title = {Evaluation of Lactic Acid Bacteria for the Control of Aflatoxin Contamination in Silage},
journal = {Agriculture, Forestry and Fisheries},
volume = {12},
number = {1},
pages = {23-31},
doi = {10.11648/j.aff.20231201.14},
url = {https://doi.org/10.11648/j.aff.20231201.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20231201.14},
abstract = {This study evaluated the effect of lactic acid bacteria (LAB) on fermentation and aflatoxin concentrations of napier grass, whole crop green maize and whole crop dry maize plant silages, infested with aflatoxigenic mold Aspergillus flavus. The forage was chopped into 10kg portions in duplicates and inoculated with 100 ml of 106-7 CFU/ml, B2 27 Lb. plantarum/pentosus/paraplantarum (LAB1); B410Lb. plantarum/pentosus/paraplantarum (LAB 2); LAB 1 + 100 ml of cultured spores (100 cfu/ml) of mold; LAB 2 + 100 ml of cultured spores (100 cfu/ml) of mold; control 1- no addition; control 2 – mold only; control 3 – LAB 1 only; control 4 – LAB 2 only. The inoculated silage bags were sealed for 90 days and sampling done monthly to evaluate LAB, fungal counts and aflatoxin B1. Dry matter (DM), pH, crude protein (CP), neutral detergent fiber (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) were determined using AOAC methods. Mold population decreased in month one but increased thereafter. Silage type determined LAB (p = 6.2e-08) and mold (p= 3.9e-08) proliferation. Dry maize and napier grass silages favoured LAB and mold growth, respectively. LAB inhibited mold growth and aflatoxin production (p= 2.2e-04). Silage pH in the third month ranged between 4.55 – 5.67 with no smell of butyric acid. Though napier and dry maize plants showed higher nutritional qualities, they favoured mold growth and aflatoxin proliferation. Aflatoxin-inhibiting LAB starters are useful in controlling aflatoxin risk in silage.},
year = {2023}
}
TY - JOUR T1 - Evaluation of Lactic Acid Bacteria for the Control of Aflatoxin Contamination in Silage AU - Sheila Okoth AU - Leonard Wamae AU - Innocent Kariuki AU - Limbikani Matumba AU - Vesa Joutsjoki AU - Hannu Korhonen Y1 - 2023/03/16 PY - 2023 N1 - https://doi.org/10.11648/j.aff.20231201.14 DO - 10.11648/j.aff.20231201.14 T2 - Agriculture, Forestry and Fisheries JF - Agriculture, Forestry and Fisheries JO - Agriculture, Forestry and Fisheries SP - 23 EP - 31 PB - Science Publishing Group SN - 2328-5648 UR - https://doi.org/10.11648/j.aff.20231201.14 AB - This study evaluated the effect of lactic acid bacteria (LAB) on fermentation and aflatoxin concentrations of napier grass, whole crop green maize and whole crop dry maize plant silages, infested with aflatoxigenic mold Aspergillus flavus. The forage was chopped into 10kg portions in duplicates and inoculated with 100 ml of 106-7 CFU/ml, B2 27 Lb. plantarum/pentosus/paraplantarum (LAB1); B410Lb. plantarum/pentosus/paraplantarum (LAB 2); LAB 1 + 100 ml of cultured spores (100 cfu/ml) of mold; LAB 2 + 100 ml of cultured spores (100 cfu/ml) of mold; control 1- no addition; control 2 – mold only; control 3 – LAB 1 only; control 4 – LAB 2 only. The inoculated silage bags were sealed for 90 days and sampling done monthly to evaluate LAB, fungal counts and aflatoxin B1. Dry matter (DM), pH, crude protein (CP), neutral detergent fiber (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) were determined using AOAC methods. Mold population decreased in month one but increased thereafter. Silage type determined LAB (p = 6.2e-08) and mold (p= 3.9e-08) proliferation. Dry maize and napier grass silages favoured LAB and mold growth, respectively. LAB inhibited mold growth and aflatoxin production (p= 2.2e-04). Silage pH in the third month ranged between 4.55 – 5.67 with no smell of butyric acid. Though napier and dry maize plants showed higher nutritional qualities, they favoured mold growth and aflatoxin proliferation. Aflatoxin-inhibiting LAB starters are useful in controlling aflatoxin risk in silage. VL - 12 IS - 1 ER -
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